Recirculating Chillers
Sigma Aldrich Fine Chemicals Biosciences DuranR GL 45 connection sy
GL 45 screw cap with either two or three ports that have GL 14 thread. This is a flexible modular system which has four different hose diameters available 1.6 mm 3.0 mm 3.2 mm and 6.0 mm that can be connected.Sterile pressure equalization is possible through use of membrane filter.
Sigma Aldrich Fine Chemicals Biosciences Tetramethylrhodamine isoth1G
methyl Tetramethylrhodamine isoth1G
Sigma Aldrich Fine Chemicals Biosciences HellmanexTM III Special Cl1EA
Hellmanex III significantly reduces the surface tension of water. The removal of dirt particles is also assured by the good wetting action of a Hellmanex III aqueous SOLUTIONon while its high emulsifying and dispersing capabilities prevent the redeposition of the loosened particles. Special surface-active substances facilitate the residue-free rinsing of the optical components once they have been cleaned.Hellmanex III is filtered to 1 mum and is therefore virtually free of solid particles.
Sigma Aldrich Fine Chemicals Biosciences RNAzolR RT for processing
RNAzol RT is a quick and convenientreagentfor use in the single-step isolation of total and small RNA from biological samples of human animal plant yeast bacterial and viral origin. A convenient single-step liquid phase separation results in the isolation of RNA from DNA protein polysaccharides and other molecules. RNAzol(R) RT can be used to isolate separate fractions of mRNA and micro RNA or to isolate total RNA containing all classes of RNA in a single fraction. This product a mixture of guanidine thiocyanate and phenol in a monophase SOLUTIONon effectively dissolves DNA RNA and protein on homogenization or lysis of tissue sample. Addition of water to the mixture allows for the precipitation of DNA proteins polysaccharides and other molecules which can then be removed by centrifugation. RNA can then be isolated by alcohol precipitation washing and solubilization. Chloroform-induced phase separation is not necessary. One mL of RNAzol(R) RT is sufficient to isolate RN
Sigma Aldrich Fine Chemicals Biosciences Denaturation Solution for
Sigmas denaturing SOLUTIONon is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination SOLUTIONon treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination allowing for more efficient transfer of large DNA in neutral Southern transfers.
Sigma Aldrich Fine Chemicals Biosciences Tetramethylrhodamine isoth1G
methyl Tetramethylrhodamine isoth1G
Sigma Aldrich Fine Chemicals Biosciences Cardiolipin sodium salt fr10MG
Cardiolipin is localized in the inner membrane of mitochondria. It contains 18 carbon atoms 3-phosphatidyl groups connected via a glycerol bridge. Cardiolipin is an important component of ATP synthase enzyme.
Sigma Aldrich Fine Chemicals Biosciences TrizmaR hydrochloride buff1L
hydrochloride TrizmaR hydrochloride buff1L
Sigma Aldrich Fine Chemicals Biosciences Hydrocortsone-Water Solub100MG
Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.
Sigma Aldrich Fine Chemicals Biosciences Chitinase from Streptomyce
Chitinase is an extracellular complex of enzymes that degrade chitin. Chitin is a cell wall component of fungi and exoskeletal essentials of different organisms which reshape their own chitin or digest/dissolve the chitin of other organisms (insects fungi yeast and algae and in the internal structures of other vertebrates). Chitinases have been detected in many microorganisms and in plants. In fungi chitinases assist in morphogenesis to break down the inherent chitin content of fungal cell walls. Plant chitinases help in resistance to fungal attack and counteracting fungal growth by targeting those same fungal cell walls. In bacteria bacterial chitinases assist in utilizing chitin as a carbon source and as an energy source. Streptomyces griseus produces multiple chitinases of different molecular masses after growth induction with chitin as the carbon source. The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions bullnbspThe first
Sigma Aldrich Fine Chemicals Biosciences TrizmaR hydrochloride solu1L
A series of Pre-mixed SOLUTIONons of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy /- 0.1 pH units.
Sigma Aldrich Fine Chemicals Biosciences AeraSealTM film non-steril
A 140 mum thick hydrophobic porous sealing film with medical-grade adhesive for tissue culture plates bio-blocks and 96-well plates where gas exchange is necessary for cell or bacterial growth. AeraSeal(TM) sealing films minimize cross-contamination spillage and evaporation. They allow uniform air and CO2 exchange for all wells unlike plate lids which favor exchange for wells near the plate edges. Dimensions 82.6 by 142.9 mm for standard-size tissue culture platenon-cytotoxic highly gas permeable easily pierceable with pipet tips or pipets for sample recovery recommended for temperatures from -20 C to 80 CNote AeraSeal(TM) films areAsuitable for hypoxic culture conditions.
Sigma Aldrich Fine Chemicals Biosciences Tetramethylrhodamne soth100MG
methyl Tetramethylrhodamne soth100MG